Journal: Iranian Journal of Parasitology

Article Title: Characterization of Specific RAPD Markers of Virulence in Trichomonas vaginalis Isolates

doi:

Figure Lengend Snippet: A) RAPD banding patterns obtained using primers Tv-5, OPA-6, and OPA-11. In square the RAPD marker used for sequencing. The arrows indicate the RAPD marker position. B) Agarose gel electrophoresis 2 % of PCR for the detection of the LRR family protein gene in T. vaginalis. The clinical presentation, RAPD cluster group and TVV infection of each isolates are shown on top. The isolate names are shown on top of each gel. MM 1 : Molecular weight marker 1 kb (Promega, USA). MM 2 : Molecular weight marker Gene Ruler™ 100 bp Plus DNA ladder (Fermentas). CP: positive control (strain ATCC 30001), NC: negative control

Article Snippet: Also the sequences were compared with the complete genome of T. vaginalis (G3 strain American Type Culture Collection PARA-98, Human, 1973, New England) using the T. vaginalis genome database (TrichoDB, http://trichodb.org/trichodb) Sequence alignments were considered biologically significant when presenting E-values below 10 - 10 and 10 -2 for nucleotide and protein sequence, respectively ( ).

Techniques: Marker, Sequencing, Agarose Gel Electrophoresis, Infection, Molecular Weight, Positive Control, Negative Control

Journal: Iranian Journal of Parasitology

Article Title: Characterization of Specific RAPD Markers of Virulence in Trichomonas vaginalis Isolates

doi:

Figure Lengend Snippet: Sequences to produce a similarity using the BLASTn program for each virulence RAPD markers

Article Snippet: Also the sequences were compared with the complete genome of T. vaginalis (G3 strain American Type Culture Collection PARA-98, Human, 1973, New England) using the T. vaginalis genome database (TrichoDB, http://trichodb.org/trichodb) Sequence alignments were considered biologically significant when presenting E-values below 10 - 10 and 10 -2 for nucleotide and protein sequence, respectively ( ).

Techniques: Marker, Virus, Sequencing, Variant Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles

doi: 10.1007/s00018-021-04083-3

Figure Lengend Snippet: Attachment of TvEpNEO and TvVPS32 parasites to NhPRE1 cells. a TvEpNEO and TvVPS32 parasites were labeled with Cell Tracker Blue CMAC (Invitrogen). Labeled parasites were then incubated for 30 min with NhPRE1 prostate cell monolayers grown on coverslips in 24-well plates at 37 °C and 5% CO2. Coverslips were washed to remove non-adherent parasites, mounted, and visualized by fluorescence microscopy. b Adherence of TvEpNEO (red bar) and TvVPS32 (green bar) parasites to NhPRE1 cells. Data are from three experiments performed in triplicate and show the fold increase in the number of parasites attached per coverslip standardized using TvEpNEO parasites with SD (p < 0.05). c Schematic representation of the experimental design. EVs from TvEpNEO (red) and TvVPS32 (green) were isolated. NhPRE1 cells were pre-incubated with increasing amount of EVs. Then, parasite attachment was analyzed by fluorescent microscopy. d Preincubation of NhPRE1 cells with VPS32-EVs or EpNEO-EVs increases adherence of a poorly adherent T. vaginalis strain (G3). Note that a stronger effect in increasing parasite adherence is observed when the cells are pre-incubated with EVs isolated from parasites transfected with VPS32. Statistical analyses were performed by comparing the adherence values of each treatment against the control treatment (*p < 0.05, **p < 0.01, ***p < 0.001). Besides, statistical analyses within each treatment were performed comparing between EVs concentration

Article Snippet: Parasites, cell cultures and media Trichomonas vaginalis strain G3 (ATCC PRA-98; Kent, UK) was cultured in TYM medium supplemented with 10% horse serum and 10 U/ml penicillin [ 27 ].

Techniques: Labeling, Incubation, Fluorescence, Microscopy, Isolation, Transfection, Control, Concentration Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles

doi: 10.1007/s00018-021-04083-3

Figure Lengend Snippet: Analysis of EVs uptake by prostate cells. a T. vaginalis VPS32-EVs or EpNEO-EVs were labeled with PKH67 dye and incubated with NhPRE1cells for 3 h. EV binding was visualized using fluorescence microscopy by incubating NhPRE1 cells with PKH67-labeled EVs or PKH67-labeled PBS as control (green). The nucleus was stained with DAPI (blue). The images are representative of 20 images viewed under similar conditions. b Quantification of EVs binding. Data shown represent the mean ± SD from 3 independent experiments, each performed in duplicate. The maximal fluorescence after incubating with labeled EVs was arbitrarily set at 100%. c Effect on uptake by temperature that block endocytosis of fluorescently labeled VPS32-EVs or EpNEO-EVs detected using flow cytometry. T. vaginalis VPS32-EVs or EpNEO-EVs were labeled with PKH67 dye, incubated with NhPRE1 cells at 37 °C or 4 °C for 45 min and uptake was quantified by flow cytometry. d Quantification of VPS32-EVs or EpNEO-EVs uptake at 37 °C or 4 °C. The maximal fluorescence after incubating with labeled VPS32-EVs was arbitrarily set at 100%. e T. vaginalis VPS32-EVs or EpNEO-EVs were labeled with PKH67 dye, incubated with NhPRE1cells for 45 min at 37 °C and the binding quantified using flow cytometry. Three independent experiments were performed. A representative experiment is shown. f Quantification of PKH67-labeled EVs uptake to NhPRE1 cells using flow cytometry. The maximal fluorescence after incubating the NhPRE1 cells with labeled VPS32-EVs was set at 100%. Data are presented are the mean ± SD from three independent experiments

Article Snippet: Parasites, cell cultures and media Trichomonas vaginalis strain G3 (ATCC PRA-98; Kent, UK) was cultured in TYM medium supplemented with 10% horse serum and 10 U/ml penicillin [ 27 ].

Techniques: Labeling, Incubation, Binding Assay, Fluorescence, Microscopy, Control, Staining, Blocking Assay, Flow Cytometry

Journal: Applied and Environmental Microbiology

Article Title: Dynamic Mechanisms of the Bactericidal Action of an Al 2 O 3 -TiO 2 -Ag Granular Material on an Escherichia coli Strain

doi: 10.1128/aem.01950-15

Figure Lengend Snippet: Figure 1. (a) Kinetics of E. coli removal by Al2O3-TiO2-Ag (16 g.L-1), (b) control kinetics: E. coli 474

Article Snippet: 96 5 97 Cultivation of bacteria, preparation of bacterial suspensions and counting 98 A non-pathogenic Escherichia coli strain (K12 DSM 423, from DSMZ, Germany) was 99 chosen as a microorganism surrogate of microbial contamination.

Techniques: Control